Pathology (Labs)
The Department of Pathology at KEM Hospital, Pune aims to provide the entire spectrum of
pathology services, education and research with a commitment to quality and excellence. We
have a state-of-the-art NABL accredited / IPAQT certified Laboratory.
Our strengths are strong interdepartmental collaboration, sensitive consultation of patient needs
and continuous search for new knowledge. Our team is an essential contributor to health care,
research, and teaching missions. Our services include: clinical pathology, cytopathology, clinical
biochemistry and endocrinology, clinical microbiology and serology, haematology, and
histopathology and cytology.
Our Unit is equipped with an auto-analyser, immune-analyser and PCR Unit. In addition to
routine biochemical tests, we are a referral centre for diagnosis of Inborn Errors of Metabolism.
Services
- Protein Electrophoresis on automatic capillary Electrophoresis by Sebia
- Immunofixation
- ADAMTS 13 Activity
- Inhibitor screen for ADAMTS 13
- Anti factor H antibody
- Complement Factor H
- C3 Nephritic Factor
- AH50
- CH50
- Posaconazole
- Interleukin 6
- Galactomannan
- TB Gold/ IGRA
Our Unit is equipped with almost all the automated systems like Vitek 2 compact, BacT/Alert 3D,
FX 40, MIGT for the rapid isolation and identification of pathogenic bacteria, fungi and
mycobacteria. The Microbiology unit is actively involved in various research projects.
Besides the routine microbiological testing, we perform special tests like Anti-Complement
Factor H assay and Endotoxin level detection test (R.O water).
Services
- Culture and sensitivity of clinical specimens, serological detection of HIV, HBsAg, HCV,
Anti HBs, Syphilis, and infectious diseases - Dengue (Antigen/Antibodies), Leptospira IgM, Rickettsia IgM, Stool for C. difficile toxin
and antigen, and Latex Agglutination test for bacterial meningitis are some of the special
facilities provided by the department
Besides the routine haematological work up, we perform special investigations for all cases of
anaemias and coagulation factor assays which are useful in the diagnosis and treatment of
various bleeding and clotting disorders, such as, haemophilia, thrombophilia, thrombocytopenia
and thrombotic disorders.
Other Services
- Diagnosis of Leukaemia including cytochemistry, (LAP Score, PAS, Peroxidase, NSE
and markers) - Platelet Aggregometer to diagnose rare platelet function defects such as Glanzmann’s
Thromboasthenia, Bernard Soulier Syndrome and Von Williebrand’s Disease - Bone Marrow Aspirations and Biopsies
The Histopathology and Cytology Unit offers complete services for all surgical pathology and cytology needs including:
- Routine Histopathology
- Intra-operative frozen section consultation for surgical and neuropathology
- Special Stains for collagen, copper, elastic tissue, calcium, amyloid, fat, fibrin, iron, melanin, mucin and microbiological organisms including fungi and various bacteria.
- Immunohistochemistry: We offer a wide range of markers (>90) including:
- Breast cancer panel: ER/PR/ HER2neu/ Ki67/ e-cadherin
- CUP Panel: For diagnosis of carcinoma of unknown primary
- Sarcoma Panel
- Lymphoma Panel: Subtyping and prognostication of common and rare lymphomas including T cell lymphomas, EBV associated lymphomas and cutaneous lymphomas.
- Viral markers: CMV, Adenovirus, SV40, EBV etc.
- MSI panel: For assessment of mismatch repair proteins in colorectal carcinomas
- Newer markers for membranous nephropathy: PLA2r, THSD7A, NELL1, EXT1/2, SEMA3B and C4d (for transplant biopsies)
- Markers for Glioma: ATRX, IDH1, P53 etc.
- Immunofluorescence on fresh tissue as well as paraffin blocks. Panel of antibodies for bullous skin disorders, vasculitis and renal lesions include IgG, IgA, IgM, C3,C1q, C4d, kappa and lambda.
Our sub-speciality sevices include oncopathology, nephropathology, dermatopathology, hepatobiliary pathology, genito-urinary pathology, neuropathology, lymphoma pathology and thoracic pathology.
We are also a centre of excellence for Digital Pathology; equipped with facilities for photography, telepathology and related research. Through this facility, we conduct online lectures for post graduate students and early career faculty once in a fornight, conduct weekly dermatopathology, GI pathology and nephropathology meetings etc.
The Cytology Unit provides preparatory and diagnostic services for all body fluid, smear and aspiration specimens. The service also provides pathologist-performed FNA (guided as well as direct) service. We have ROSE (rapid on site examination) for endoscopic ultrasound guided biopsies (EUS) and endobronchial ultrasound guided biopsies (EBUS). Services by liquid based cytology for gynaecology / non-gynaecology samples are also available. Cell block preparation with immunohistochemistry is a routinely performed procedure in our laboratory for all cases which are positive for malignant cells.
